Sleep deprivation leads to a repertoire of cognitive, attentional and emotional deficits that are seriously detrimental in occupations requiring alertness. These deficits may be associated with changes in gene expression in specific regions of the brain.

The Allen Institute is in the process of generating a collection of cellular resolution data for gene expression in the mouse brain in response to sleep deprivation and sleep state. Using the Allen Institute high throughput in situ hybridization platform, gene expression has been examined under five experimental conditions in mice in collaboration with SRI International, and the images are presented online as a resource for sleep researchers. Sleep conditions are as follows:

Code	Condition
SD	Sleep-deprived for 6 hours (ZT0-ZT6)
SD-C	Time of day control for sleep-deprivation (ZT6)
RS	Recovery sleep (4 hours) following 6 hours of sleep deprivation (ZT6-ZT10)
RS-C	Time of day control for recovery sleep (ZT10)
W	Waking (ZT18)

Project data is available in the following formats:

• Colorimetric ISH and Nissl images for 224 genes across 5 conditions, some with replicates
• Microarray data for 7 brain regions across 5 conditions
• Difference grids for viewing differences between conditions in 3-D using Brain Explorer

For more details, please refer to the documentation.

This work was funded by the Department of Defense USAMRAA award W81XWH-06-1-0131.